Antibody Engineering

A guide to LabGene library selection for antibody engineers

 
 

Hit generation and lead selection

Precision combinatorial

 

Lead optimisation and characterisation

Cost effective combinatorial

 

 
 
 

 

 
 
 
 

Hit generation and lead selection

 
 

Our most advanced combinatorial libraries. Precise and controlled coverage of large areas of sequence space. Maximise the probability of generating high quality hits.

 
 
 

LabGene Combi X

Codon-controlled combinatorial libraries.

 
 

Codon control

Choose any subset of amino acids with defined ratios, at any degenerate position. Minimise out of frame mutations and eliminate stop codons. Control codon composition down to 0.5% representation at any individual position for any given amino acid (e.g. Ser = 81%, Thr = 5%, Asn = 4%, Lys = 9.5%, Glu = 0.5%). Use of entire IUPAC code.

CDR coverage

Vary all three CDRs simultaneously with up to 3 x 150 bp degenerate regions. 

 

Diversity

Up to 10^13 variants, whether cloned or linear. Every variant is unique.

Turnaround time

Libraries shipped in as little as 8 weeks, with more complex libraries in up to 16 weeks.

 

 
 
 

 

 
 
 

Lead optimisation and characterisation

 
 
 
 

LabGene Combi S

Precise ratio control.

 
 

Control

LabGenius library design algorithms to determine optimum degenerate codon usage and DNA mutation rate.

Minimise biasing and stop codon occurrence. Optimise for desired diversity at each position. Closely match mutation rate at the amino acid level (either overall mutation rate, or position by position). Control nucleotide distribution with ratios of individual bases down to 0.5% for a given position.

Design

Up to 450 bp of degeneracy in 3 x 150 bp degenerate windows. Degenerate windows separated by constant regions of at least 30 bp. Use of entire IUPAC code.

Price

From £1,998 ($2,998).

Turnaround time

From 3 weeks. 

 
 
 

LabGene Combi C

Cost effective, rapid turnaround combinatorial libraries.

 
 

Optimise your lead antibody binding characteristics with LabGene Combi C combinatorial libraries.

Flexible design

Simultaneously modify up to 450 bp bases in 3 x 150 bp degenerate windows. Degenerate windows separated by constant regions of at least 30 bp. Use of entire IUPAC code. LG design algorithms for optimising amino acid choice with degenerate bases.

Diversity

Up to 10^13 variants, whether cloned or linear. Every variant is unique.

Cost effective

From £999 ($1,499).

Rapid turnaround

Dispatched in as little as 15 - 20 business days.

 
 
 

 

Applications summary

 

Library
Combi C
Combi S
Combi X
Hit generation and lead selection
X
X
Affinity maturation
X
X
Fc engineering
X
X
X
Humanisation
X
X
 

 
 

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