LabGene DNA Libraries

Accelerate evolution

 

Combinatorial libraries for precise and controlled coverage of large areas of sequence space. Maximise the probability of generating high quality hits.

 
 
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LabGene Combi S

Precise ratio control.

 
 

Control

LabGenius library design algorithms to determine optimum degenerate codon usage and DNA mutation rate.

Minimise biasing and stop codon occurrence. Optimise for desired diversity at each position. Closely match mutation rate at the amino acid level (either overall mutation rate, or position by position). Control nucleotide distribution with ratios of individual bases down to 0.5% for a given position.

Design

Up to 450 bp of degeneracy in 3 x 150 bp degenerate windows. Degenerate windows separated by constant regions of at least 30 bp. Use of entire IUPAC code.

Price

From £1,998.

Turnaround time

From 3 weeks. 

 
 
 

LabGene Combi C

Cost effective, rapid turnaround combinatorial libraries.

 
 

Flexible design

Simultaneously modify up to 450 bp bases in 3 x 150 bp degenerate windows. Degenerate windows separated by constant regions of at least 30 bp. Use of entire IUPAC code. LG design algorithms for optimising amino acid choice with degenerate bases.

Diversity

Up to 10^13 variants, whether cloned or linear. Every variant is unique.

Cost effective

From £999.

Rapid turnaround

Dispatched in as little as 15 - 20 business days.

 
 
 

LabGene Combi X

Codon-controlled combinatorial libraries.

 
 

Codon control

Choose any subset of amino acids with defined ratios, at any degenerate position. Minimise out of frame mutations and eliminate stop codons. Control codon composition down to 0.5% representation at any individual position for any given amino acid (e.g. Ser = 81%, Thr = 5%, Asn = 4%, Lys = 9.5%, Glu = 0.5%). Use of entire IUPAC code.

Design parameters

Vary all three CDRs simultaneously with up to 3 x 150 bp degenerate regions. 

Diversity

Up to 10^13 variants, whether cloned or linear. Every variant is unique.

Turnaround time

Libraries shipped in as little as 8 weeks, with more complex libraries in up to 16 weeks.

 
 

 

Summary

Comparison to other library generation methods

Our patented assembly method gives us three key advantages over other methods. No PCR amplification is used, which minimises biasing of the final library. We use no long-sequence hybridisation, ensuring a high fidelity and allowing for greater control over where degeneracy is positioned. Finally, our high efficiency allows us to introduce unprecedented levels of diversity into the final pool.

Approach to library generation Diversity Fidelity Control Production time Value Score
LabGenius +++ ++ +++ ++ +++ 13/15
Mutagenic strains + - - - +++ 4/15
Error-prone PCR + ++ - +++ +++ 9/15
Chip-based oligo synthesis ++ + +++ + +++ 10/15
Double-stranded enzymatic synthesis ++ +++ +++ - + 9/15
Single stranded overlap assembly ++ + +++ + ++ 9/15
Off the shelf Ab libraries ++ +++ + +++ + 10/15
 

 
 

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